To help you photo regions usually involved in the hereditary power over leaf corrosion opposition, the fresh new QTL-evaluation thickness list (Chardon et al. 200cuatro) is actually calculated (Fig. step step 3). It figure develops inside an area having (i) what number of QTL away from more studies, (ii) the new proximity anywhere between QTL ranking, and (iii) the precision of the individual QTL updates quote.
Fig. step 3. Brand new analysis statistic is computed all 1 centimorgan (cM) and plotted collectively per chromosome (blue bend). Two lateral dotted outlines are the average value of the latest statistic (0.055) together with threshold having higher philosophy (0.2seven5), recognized as 5 times the typical well worth (Chardon ainsi que al. 2004). Bars beneath the evaluation curve represent the newest wheat chromosomes where QTL was in fact projected. Light gray (green) packets represent meta-QTL harboring no less than 2 QTL, while dark-gray (red) packets depict unique QTL. Ranking away from in earlier times mapped Lr genes inside the wheat are portrayed by triangles lower than chromosome bars; Lr genetics colocalized with meta-QTL was indicated in the dark gray (blue).
Brand new analysis list are computed every centimorgan and you will rates have been received for each of one’s 144 estimated QTL (Fig. 3). As a whole, 52 overview highs were received, of which 47 surpassed the average value of the latest fact (0.055) over the genome and you may illustrated genomic beautiful locations to own leaf rust opposition. Of 47 highs, twenty five presented thinking surpassing this new highest-well worth endurance (0.275) (Fig. 3). Just how many highs exceeding the common endurance varied from just one when you look at the chromosome 4A and you may 7A to help you four when you look at the chromosomes 1B, 2D, and 7B. On latter, all five peaks as well as exceeded the fresh highest-worthy of endurance.
QTL meta-study.
The 144 QTL projected on the Wheat Ingredient 2004 map was basically subjected to meta-study by using the qualities of Goffinet and you can Gerber (2000) if the amount of QTL within the an excellent chromosome was below 10 and people regarding Veyrieras mais aussi al. (2007) in the event the matter are 10 or higher. Following the a keen AIC, 141 QTL was in fact labeled toward forty eight MQTL (Dining table 2). The software program failed to imagine about three QTL getting meta-analysis for their lower LOD get values otherwise high Si. In most, thirty-five MQTL, spanning 128 QTL, had been caused by the fresh new consolidation with a minimum of 2 QTL, and you may thirteen QTL stayed just like the book QTL (Table 2).
a your higher a portion of the table reveals MQTL spanning within minimum a few clustered QTL and also the lower region suggests QTL leftover due to the fact unique QTL.
c AUDPC = area according to the problem advances bend, DS = disease seriousness, EA = very early aborted territories, EST = created colonies, Web browser = problems abilities, They = infection type, LIA = leaf contaminated city, LP = hidden months, LR = leaf resistance, LS = lesion size, LTN = leaf tip necrosis, NEC = necrotic colonies, SPL = spore development for every single lesion, and you will SPS = spore creation for every single tool from sporulating tissue.
| Chromosome | MQTL | Standing (cM) | Si (nine5%) | Flanking indicators | QTL (n) b | Characteristic c | Opposition provider |
|---|---|---|---|---|---|---|---|
| 1A | MQTL1 | Xfba254–Xwmc95b | 4 | AUDPC, DS | Apache, Sujata | ||
| 1B | MQTL2 | step one.8six | Xcdo98.2–Xmwg837b | 6 | AUDPC, DS, They, LTN | Forno, MG5323, Oberkulmer, Pastor, Sujata | |
| MQTL3 | 8.46 | Xbarc81–Xfba294 | 2 | AUDPC, DS | Bainong 64 | ||
| MQTL4 | 2.ten | Xfba266c–XksuI27 | 2 | DS | Pastor, Pavon 76 | ||
| MQTL5 | 0.twenty-eight | XksuI27–Xbarc80 | 2 | AUDPC, DS | Francolin#1 | ||
| 2A | MQTL6 | 4.62 | Xglk554–Xcmwg682b | 2 | AUDPC | Colosseo, Forno | |
| 2B | MQTL7 | step 3.68 | Xgwm55–Xgwm429 | 4 | AUDPC, DS, It | Carberry, Kariega, Avocet S, W-7984 | |
| MQTL8 | Xwmc265–Xgwm877.1 | 7 | AUDPC, DS, LP | Apache, Creso | |||
| 2D | MQTL9 | nine.32 | Xgwm455–Xbcd718 | 3 | AUDPC, DS | Equilibrium, Forno, MN99394-1 | |
| MQTL10 | 8.forty | Xfba74a–Xgwm30.1 | 3 | AUDPC, DS, LTN | Forno, Pastor | ||
| MQTLeleven | cuatro.eleven | Xcdo1008–Xksud23a | 6 | AUDPC, DS, SPS | Balance | ||
| 3A | MQTL12 | 6.73 | Xcdo1345–Xgwm2 | 2 | LR, LTN | Forno, Oberkulmer | |
| MQTL13 | Xcfa2134–XgbxG242 | 2 | They, LIA | TA4152-sixty | |||
| MQTL14 | 2.32 | Xmwg570–Xcdo113 | 2 | AUDPC, LIA | Lloyd | ||
| 3B | MQTL15 | 2.08 | XksuG30a–Xgwm533a | 9 | DS, Web browser, It, LIA, LP, SPL | Balance, Capo, Opata 85 | |
| MQTL16 | 0.89 | Xgwm566–Xgwm888.step 3 | 3 | DS, It, LIA | Francolin#step one, TA4152-60 | ||
| 4A | MQTL17 | XgbxR247–Xpsr593.dos | 2 | DS, It | Opata 85 | ||
| 4B | MQTL18 | Xgwm495a–Xcsu25 | 3 | AUDPC, DS | Avocet S, Creso, Forno | ||
| MQTL19 | Xgwm131.2–Xmwg11a | 2 | DS, LTN | Carberry, Oberkulmer | |||
| 4D | MQTL20 | 2.fifty | Xbarc98–Xfbb336 | 3 | AUDPC, It, LIA | Sujata, TA4252-60 | |
| MQTL21 | Xcdo1312a–Xfba177b | 4 | AUDPC, DS, LS, SPL | Balance | |||
| 5B | MQTL22 | cuatro.79 | Xbarc4–Xgwm371 | 2 | AUDPC, DS | Kariega, SHA3/CBRD | |
| MQTL23 | 8.83 | Xpsp3065–Xgwm118 | 5 | They, LIA, LTN | Americano 25e, Capo, Carberry, TA4152-60 | ||
| 6A | MQTL24 | Xmwg67.1–XksuH4.dos | 6 | AUDPC, DS, LP | Balance | ||
| 6B | MQTL25 | S25M19_7–Xcdo476.2 | 2 | AUDPC, DS | Bainong 64 | ||
| MQTL26 | Gpc-B1–Xwmc388a | 2 | AUDPC, DS | Bainong 65 | |||
| MQTL27 | Xgwm133b–Xfbb130 | 2 | DS, They | Pavon 76, TA4152-sixty | |||
| MQTL28 | six.91 | Xbarc134–Xwmc417b | 2 | AUDPC, DS | Kariega, Pastor | ||
| 7A | MQTL29 | cuatro.73 | XWC2028g–P78/M49.4 | 5 | AUDPC, DS | Balance | |
| 7B | MQTL30 | 3.58 | Xwmc335–Xgwm68.dos | 5 | AUDPC, DS, LIA | Colosseo, Forno, Kariega, SHA3/CBRD | |
| MQTL31 | dos.17 | S25M15_19–Xgwm112 | 4 | DS, They, LTN | MG5323, Oberkulmer | ||
| MQTL32 | cuatro.42 | Xwmc273c–Xwmc311 | 4 | AUDPC, DS, LTN | Forno, Oberkulmer, Opata 85, Pastor | ||
| MQTL33 | 0.53 | Xgwm577–Xbarc340.2 | 11 | AUDPC, DS, EA, EST, LIA, LP, NEC | Colosseo, Creso, Sujata | ||
| 7D | MQTL34 | 1.75 | Xbcd1438–Xcnl6b | 3 | AUDPC, DS, LTN | Forno, MN98550-5 | |
| MQTL35 | 9.39 | Xgwm44–Xcfa2174c | 2 | DS, LTN | Oberkulmer, Opata 85 | ||
| 1A | … | 123.5 | Xwmc237–XksuE11.1a | 1 | DS | SHA3/CBRD | |
| 1B | … | six.13 | XksuF43.1–Xabc155c | 1 | LIA | Capo | |
| 2A | … | Xfbb329–Xgwm339 | 1 | LP | Creso | ||
| … | step 3.09 | Xfbb353.2–Xglk554 | 1 | LIA | Colosseo | ||
| 2B | … | cuatro.98 | Xwmc344–XksuD22 | 1 | LIA | Capo | |
| … | 4.twenty-seven | Xfba310.1–Xbcd1231 | 1 | LIA | Capo | ||
| 2D | … | Xwmc243–Xgwm608.step 3 | 1 | DS | SHA3/CBRD | ||
| 5A | … | 2.00 | 5.55 | Xcnl6b–Xgwm154a | 1 | LTN | Oberkulmer |
| … | Xbcd508–Xfbb255x | 1 | DS | Pastor | |||
| … | Xwg114a–P43/M62-5 | 1 | EA | Creso | |||
| 6A | … | 9.88 | Sr26–P38/M51-cuatro | 1 | LTN | Oberkulmer | |
| 7D | … | step three.forty eight | XgbxG564–Yr18 | 1 | DS | Opata 85 | |
| … | Xgwm121–Xbarc53 | 1 | LTN | Carberry |